Adaptive growth of AZA-R cells showing decreased expression of UCK2 in vitro but not in vivo. (A) Immunoblots showing amounts of protein in each cell line after treatment for 4 days with 100 nM AZA. (B) The number of live cells was measured by trypan blue staining. Blue line, parental cells; red line, AZA-R cells. The results are expressed as the mean ± SD of 3 independent experiments. ∗P < .05. (C) The number of migrated cells was counted after staining with trypan blue. Migration was induced by incubation for 24 hours with FBS. The results are expressed as the mean ± SD of 3 independent experiments. (D-E) Tumor development and size in Balb/c Rag-2^−/− Jak3^−/− mice injected subcutaneously with parental cells (blue) or AZA-R (red) TL-Om1 and MT-2 cells. Tumors measuring more than 5 mm in diameter were counted. (F) TL-Om1 tumors were isolated from mice sacrificed on day 39, and MT-2 tumors were isolated from mice sacrificed on day 25. FBS, fetal bovine serum.