Reprogramming of pyrimidine nucleotide biosynthesis in AZA-R cells showing reduced expression of UCK2. Each metabolite (except for CoQ10) of TL-Om1 AZA-R cells (red) cultured under normal growth conditions was identified and compared with those of TL-Om1 parental cells (blue) using the ω-Scan package (HMT). Both oxidized and reduced CoQ10 present in whole cell extracts was measured as described in “Materials and methods.” CDA, Cytidine deaminase; CMP, Cytidine monophosphate; CMPK, Cytidine monophosphate kinase; NDPK, Nucleoside diphosphate kinase; UMP, Uridine monophosphate.