Figure 5.
Pevonedistat induces apoptosis and cell cycle arrest in ATLL cell lines. (A) Viable cell numbers measured by the MTS assay for ATLL cell lines treated with the indicated amount of pevonedistat for 96 hours. (B) The percentages of apoptotic cells in KK1 cells treated with or without pevonedistat at 1250 nM for 48 hours were detected by analyzing annexin V and propidium iodide (PI) on flow cytometry. (C) The percentage of PI(−) annexin V(+)/PI(−) KK1 cells treated with or without pevonedistat at 1250 nM was monitored at 48 and 96 hours. The black and red bars represent DMSO- or pevonedistat-treated cells, respectively. (D) DNA content was analyzed in KK1 cells with or without pevonedistat for 48 hours. Error bars represent the mean with SEM of replicates. ∗∗∗P < .001, Welch 2-sample t test. All experiments were repeated at least twice.

Pevonedistat induces apoptosis and cell cycle arrest in ATLL cell lines. (A) Viable cell numbers measured by the MTS assay for ATLL cell lines treated with the indicated amount of pevonedistat for 96 hours. (B) The percentages of apoptotic cells in KK1 cells treated with or without pevonedistat at 1250 nM for 48 hours were detected by analyzing annexin V and propidium iodide (PI) on flow cytometry. (C) The percentage of PI(−) annexin V(+)/PI(−) KK1 cells treated with or without pevonedistat at 1250 nM was monitored at 48 and 96 hours. The black and red bars represent DMSO- or pevonedistat-treated cells, respectively. (D) DNA content was analyzed in KK1 cells with or without pevonedistat for 48 hours. Error bars represent the mean with SEM of replicates. ∗∗∗P < .001, Welch 2-sample t test. All experiments were repeated at least twice.

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