Emerging erythroid cells exhibit functionally distinct stages along the differentiation trajectory. (A) UMAP plot of erythroid cell populations colored by pseudotime. (B) Violin plot showing the distribution of pseudotime in all initial erythroid cell population clusters. (C) Pseudotime trajectory analysis demonstrating the directions of erythroid differentiation as indicated by the arrows along various erythroid populations. (D) UMAP visualization of the 3 regrouped stages in the emerging erythroid cells in the pooled scRNA-seq data. (E) UMAP visualization of the various stages of cell cycle in the pooled scRNA-seq data. (F) Bar graph showing the proportions of different cell-cycle phases (G1, S, and G2M) of pooled spleen cells from different stages. (G) Dot plot demonstrating the enrichment analysis (GO biological processes) of DEGs from stages 1, 2, and 3, respectively. (H) Expressions of selected genes in all erythroid populations along the pseudotime axis.