Transcriptomic analysis of steady state BM single cells. (A) UMAP visualization of scRNA-seq data from steady state BM samples, with annotations of 11 subpopulations. (B) UMAP visualization of the 5 reclustered groups in the erythroid progenitor populations in steady state bone marrow scRNA-seq data. (C) UMAP visualization of the selected gene expression levels among various groups in steady state BM erythroid progenitors, with Group_2 highlighted in ovals. (D) Violin plots comparing the differences in selected gene expression levels among various groups in steady state bone marrow erythroid progenitors. (E) UMAP visualization and quantification of the distribution of “hiatus” subpopulation in steady state BM, PHZ-treated spleen and bleeding-treated spleen, respectively. (F) UMAP visualization and bar graph showing the proportions of different cell-cycle phases (G1, S, and G2M) of steady state BM cells from different stages (stages_1 to _3). (G) Dot plot demonstrating the enrichment analysis (GO biological processes) of DEGs from stages_1, 2, and 3 in steady state BM erythroid progenitors. (H) UMAP visualization of the selected gene expression levels among various stages in steady state BM erythroid progenitors. (I) Violin plots comparing the differences in selected gene expression levels among various stages in steady state BM erythroid progenitors. NK cell, natural killer cell; SRP, signal-recognition particle.