Relationship between BM neutrophil lineage development and G-CSF responsiveness in different patient groups. (A) bANC before G-CSF treatment and pANC after G-CSF treatment in 36 patients who underwent concurrent G-CSF therapy and ANC monitoring. pANC, peak ANC levels within 10 days after the initiation of G-CSF. Student t test was used for comparation of ANC before and after G-CSF treatment within each group. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. Bars represent means ± SD. (B) Statistical analysis of the correlation between the proportion of BM neutrophilic lineage cells and peripheral pANC, using Spearman correlation test. (C) Detailed analysis of the percentage of BM neutrophilic lineage cells and peripheral pANC in 30 patients who received G-CSF treatment concomitant with a cell morphology assay of BM smears. (D) Correlation between pANC and the populations of stem cell pool (myeloblast), the mitotic cell pool (promyelocyte, myelocyte) and the postmitotic cell pool (metamyelocyte, band cell and PMN), following the criteria described by Hong et al,²⁴ using Spearman correlation test.