The myeloid gene signature is related to the presence of lymphocyte-monocyte complexes in LKs. (A) Frequency of monocytes evaluated by flow cytometry in the LKs of patients expressing the 4-gene signature (SIG^pos) or not (SIG^neg), n = 38. (B) Representative density plots showing CD3⁺ and CD14⁺ cells (among singlets, viable, and CD3⁺ cells), in an unselected LK (left) and in a MACS-selected CD3⁺ selected LK (right). (C) Percentage of CD3⁺ CD14⁺ cells, evaluated by MFC, among CD45⁺ viable cells in unsorted LKs of SIG^pos and SIG^neg (n = 30). (D) Random gallery of events for CD3⁺ T cells, CD14⁺ monocytes, and doublets/triplets expressing both CD3 and CD14, in unsorted LKs visualized using DEPArray PLUS image-based platform. 4′,6-diamidino-2-phenylindole (DAPI) was used to stain nuclei. Experiments were performed using samples belonging to 3 different patients. Exact median values are reported. In panels A and C, comparisons were made by the Mann-Whitney test (∗P < .05; ∗∗∗P < .001). LK, leukapheresis product.