High levels of circulating AMC significantly reduced survival of patients expressing the signature. (A) Kaplan-Meier curve of PFS in patients stratified according to PB AMC levels at the time of leukapheresis and to the 4-gene signature (n = 32). The median value of AMC (360/μL) was used to dichotomize patients. SIG^pos/high-AMC, patients expressing the signature and displaying circulating AMC above the median (n = 20). SIG^pos/low-AMC, patients expressing the signature and displaying circulating AMC below the median (n = 12). (B) Kaplan-Meier curve of PFS in patients stratified according to CRP levels at the time of LK and to the 4-gene signature (n = 28). CRP upper normal limit (ULN) was used to dichotomize patients. SIG^pos/high-CRP, patients expressing the signature and displaying CRP levels above the ULN (n = 19). SIG^pos/low-CRP, patients expressing the signature and displaying CRP levels below the ULN (n = 9). (C) Kaplan-Meier curve of PFS in patients stratified according to the percentage of circulating lymphocytes at the time of leukapheresis and to the 4-gene signature (n = 32). The median value of the percentage of lymphocytes (21.6%) was used to dichotomize patients. SIG^pos/high % lympho, patients expressing the signature and displaying lymphocytes levels above the median (n = 10). SIG^pos/low % lympho, patients expressing the signature and displaying lymphocytes levels below the median (n = 22). Comparisons were made applying the log-rank test.