Figure 1.
DICER1 depletion induces HBG partially by upregulation of HIC2. (A) Western blot with indicated antibodies of extracts from DICER1 depleted HUDEP2 cells. Nontargeting sgRNA was used as control. (B) HBG and BCL11A mRNA measured by qRT-PCR in DICER1 depleted HUDEP2 cells. Results are normalized to AHSP and shown as mean ± SD (2 independent biological replicates with 2 technical replicates each). (C) Western blot with indicated antibodies of extracts from DICER1 and HIC2 depleted HUDEP2 cells. (D) HBG and BCL11A mRNA measured by qRT-PCR in DICER1 and HIC2 depleted HUDEP2 cells. Results are normalized to AHSP and shown as mean ± SD (2 independent biological replicates with 2 technical replicates each). (E) Schematic demonstrating repression of HIC2 in adult cells by miRNAs. Ablation of miRNA processing leads to increased HIC2 expression, decreased BCL11A, and derepression of HBG. SD, standard deviation.

DICER1 depletion induces HBG partially by upregulation of HIC2. (A) Western blot with indicated antibodies of extracts from DICER1 depleted HUDEP2 cells. Nontargeting sgRNA was used as control. (B) HBG and BCL11A mRNA measured by qRT-PCR in DICER1 depleted HUDEP2 cells. Results are normalized to AHSP and shown as mean ± SD (2 independent biological replicates with 2 technical replicates each). (C) Western blot with indicated antibodies of extracts from DICER1 and HIC2 depleted HUDEP2 cells. (D) HBG and BCL11A mRNA measured by qRT-PCR in DICER1 and HIC2 depleted HUDEP2 cells. Results are normalized to AHSP and shown as mean ± SD (2 independent biological replicates with 2 technical replicates each). (E) Schematic demonstrating repression of HIC2 in adult cells by miRNAs. Ablation of miRNA processing leads to increased HIC2 expression, decreased BCL11A, and derepression of HBG. SD, standard deviation.

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