Figure 4.
Comparative HDX of AIM-A1 protein with and without bound 6D12. (A) Relative deuterium uptake displayed on the 3D structure of the A1 domain and partial AIM (PDB 1SQ0). Residues experiencing more exchange in the presence of 6D12 are colored in shades of red, no difference in exchange is displayed as white, and decreased exchange is shown in cyan. Note that many residues in the AIM were unresolved in this structure. (B-E) Uptake plots of representative peptides in AIM-A1 with (red) and without (black) bound 6D12 at 10, 30, 100, and 1000 seconds of exchange. Peptide 1239-1252 (B) is a NAIM-peptide. Peptide 1308-1318 (C) is in the α1β2 loop of the A1 domain, peptide 1330-1340 (D) is in the β3α2 loop, and peptide 1369-1382 (E) is in the α3β4 loop. For each time point, experiments were performed in triplicate. ∗∗∗∗P < .0001 using a Student t test; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.

Comparative HDX of AIM-A1 protein with and without bound 6D12. (A) Relative deuterium uptake displayed on the 3D structure of the A1 domain and partial AIM (PDB 1SQ0). Residues experiencing more exchange in the presence of 6D12 are colored in shades of red, no difference in exchange is displayed as white, and decreased exchange is shown in cyan. Note that many residues in the AIM were unresolved in this structure. (B-E) Uptake plots of representative peptides in AIM-A1 with (red) and without (black) bound 6D12 at 10, 30, 100, and 1000 seconds of exchange. Peptide 1239-1252 (B) is a NAIM-peptide. Peptide 1308-1318 (C) is in the α1β2 loop of the A1 domain, peptide 1330-1340 (D) is in the β3α2 loop, and peptide 1369-1382 (E) is in the α3β4 loop. For each time point, experiments were performed in triplicate. ∗∗∗∗P < .0001 using a Student t test; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.

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