Stroke leads to increased neutrophil integrin α9, higher plasma VCAM-1 levels, and increased DVT severity. (A) Neutrophil integrin α9 and (B) plasma VCAM-1 levels from patients with stroke and healthy controls. (C) Schematic of experimental design. (D) Representative image of flow-cytometric analysis of integrin α9 for each group (left) and quantification of α9 expression in peripheral neutrophils after stroke or sham surgery in mice (right). (E) Expression of α9 relative to Actb in peripheral neutrophils after stroke or sham surgery. (F) Plasma VCAM-1 levels from mice with stroke and mice with sham surgery. (G) Schematic of experimental design for stroke-DVT studies. (H) Representative IVC thrombus harvested 48 hours after stenosis from each group (left) and thrombus weight (mg; right). Only mice that exhibited thrombosis were included to quantify the thrombus weight. Each dot represents a single mouse. (I) Thrombosis incidence. (J) Expression of α9 relative to Actb in peripheral neutrophils after DVT and stroke-DVT. Data are mean ± standard error of the mean (SEM) and analyzed using the Mann-Whitney test (A-B,D-F); Fisher exact test (I); and Kruskal-Wallis test followed by the corrected method of Benjamini and Yekutieli (J); n = 8 (A-B); n = 6 (D-F); n = 20 (H-I); and n = 4-6 (J). hr, hour; mRNA, messenger RNA.