Macitentan pretreatment reduces poststroke DVT severity. (A) Schematic of experimental design. (B) Representative IVC thrombus harvested 48 hours after stenosis from each group (left); and thrombus weight (mg; right). Only mice that exhibited thrombosis were included to quantify the thrombus weight. Each dot represents a single mouse. (C) Thrombosis incidence. (D) Representative cross-sectional immunofluorescence image (left) of the isolated IVC IVC thrombus (48 hours after stenosis) from each group for Ly6G (neutrophils, green) and DAPI (blue). magnification, 20×; scale bar, 50 μm; and quantification (right). (E) Representative cross-sectional immunofluorescence image (left) of the isolated IVC thrombus (48 hours after stenosis) from each group for the antihistone H3 (citrulline R2 + R8 + R17) (NETs, red) and DAPI (blue); magnification, 20×; Scale bar, 50 μm; and quantification (right). Data are mean ± SEM and analyzed by repeated measures ANOVA followed by the corrected method of Benjamini and Yekutieli (B,D,E) or Fisher exact test (C); n = 20 (B-C); and n = 5-6 (D-E). DAPI, 4',6-diamidino-2-phenylindole.