Figure 4.
CTBP2 reduces H3K27ac binding to MYC and IRF4 promoters in MM. (A) The Txn Factor chromatin immunoprecipitation (ChIP) track displaying CTBP2 binding sites (gray bars) on (left) MYC and (right) IRF4 loci in human ESC from the ChIP-seq data of the ENCODE project. ChIP-PCR primers were labeled with respect to their distance to the transcription start site , which was denoted as +1. (B-E) After CTBP2 transduction in NCI-H929 cells, ChIP-PCR was performed to show the (B) occupancy of CTBP2 and enrichment of (C) H3K27ac, (D) H3K4me3, and (E) H3K27me3 at the enhancer (EN) region, first exon of MYC, and the promoter and first intron of IRF4. The regions analyzed were labeled with respective to their distance from the transcription start site. GAPDH promoter and noncoding (NR) region served as a negative control. The results are expressed as the mean ± SD of triplicate measurements from at least 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗∗P < .0001.

CTBP2 reduces H3K27ac binding to MYC and IRF4 promoters in MM. (A) The Txn Factor chromatin immunoprecipitation (ChIP) track displaying CTBP2 binding sites (gray bars) on (left) MYC and (right) IRF4 loci in human ESC from the ChIP-seq data of the ENCODE project. ChIP-PCR primers were labeled with respect to their distance to the transcription start site , which was denoted as +1. (B-E) After CTBP2 transduction in NCI-H929 cells, ChIP-PCR was performed to show the (B) occupancy of CTBP2 and enrichment of (C) H3K27ac, (D) H3K4me3, and (E) H3K27me3 at the enhancer (EN) region, first exon of MYC, and the promoter and first intron of IRF4. The regions analyzed were labeled with respective to their distance from the transcription start site. GAPDH promoter and noncoding (NR) region served as a negative control. The results are expressed as the mean ± SD of triplicate measurements from at least 3 independent experiments. ∗P < .05; ∗∗P < .01; ∗∗∗∗P < .0001.

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