Examining the effects of drug treatments on advanced stages of MPN. (A) Experimental setup. (B) Spleen weight measured after 6 weeks of treatment. (C) Blood counts of treated mice before and after 6 weeks of treatment. (D) Blood glucose levels measured weekly during the treatment (left) and glucose tolerance test performed after 5 weeks of treatment (right). (E) Reticulin fibrosis grade determined in sternum after 6 weeks of treatment. (F) Frequencies of LT-HSCs in BM and their cell cycle status (left panel) and normalized percentages of LT-HSCs in different stages of the cell cycle (right panel). The markers used for cell cycle analysis were G0: Ki67⁻ DAPI^−, G1: Ki67⁺ DAPI⁻, and S/G2/M: Ki67⁺ DAPI⁺. Mean ± SEM is represented. 2-way ANOVA with multiple comparison against vehicle was performed with Fisher LSD test for panels C-D,F. 1-way ANOVA with multiple comparison to the vehicle group was performed with Fisher LSD test on panels B,E. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001.