BAHCC1 is critical for MLL-rearranged AML. (A) Gene set enrichment analysis (GSEA) of MLL-rearranged AML samples (GSE17855³⁶) with high (B1-H, n = 9) and low (B1-L, n = 9) expression of BAHCC1 using C6 oncogenic signature gene sets. GSEA plot using the most enriched gene set, MYC_UP.V1_UP,³⁸ and a list of the top 5 enriched gene sets are shown (filtered by NOM (nominal) P < .05 and FDR q < 0.25). NES, normalized enrichment score. (B) RT-qPCR of Myc in colony-forming cells derived from the Bahcc1-depleted cells shown in Figure 3C. Bar graphs show the mean with SD of at least 3 independent experiments. (C) Expression levels of BAHCC1 in AML samples (GSE19577³⁹) with MLL-AF9 (n = 11) and other MLL-fusion genes (n = 31). Data are shown in box and whisker plots. ^†P = .054; ^∗∗∗∗P < .0001; ns, not significant ANOVA followed by Dunnett multiple comparison for panel B and Mann-Whitney U test for panel C.