Conditioning Fanca−/− mice using anti-CD45-PBD resulted in significantly less acute GVHD than myeloablative TBI in MHC-mismatched transplantation. Recipient mice were evaluated for donor engraftment, clinical GVHD, and survival. (A) Chimerism of donor cells (H2Kd+) in the peripheral blood (PB) of transplanted Fanca knockout mice (129S1 strain) was analyzed at day 57 post-BMT. (B) The recipient Fanca knockout mice were evaluated for clinical GVHD and survival at day 57. BM, 10 × 106 WT BALB/c BM donor cells; BMT, bone marrow transplantation; T, 2 × 106 BALB/c purified donor T cells; TBI, conditioning by total body irradiation; CD45-ADC, conditioning by anti-CD45-PBD; isotope-ADC, conditioning by antibody isotope-PBD. Adapted from Figure 7 in the article by Saha et al, which begins on page 2201.
