QCG supplementation rapidly protects RBCs from intracellular ROS formation during oxidative stress. RBCs pretreated with either PBS, Gln (Q), or QCG were labeled with DCFDA, placed in a glass bottom petri dish per sample condition, and allowed to settle for 45 seconds before given a bolus (red arrow, A) of PBS (gray line, B-D) or 50 μM H2O2 (red line, B-D). Stationary cells (total 8 cells per condition) were quantified (A) and imaged (B-D) per condition at each 15 second time point for 5 minutes. Images were taken with a Leica Thunder at 63× oil immersion (A-D). Representative images of DCFDA labeled RBCs stressed with 50 μM H2O2 (left) or PfCM (right) (E). Images taken with Echo Revolve fluorescent microscope at 100× oil immersion (E).
Figure 6.

QCG supplementation rapidly protects RBCs from intracellular ROS formation during oxidative stress. RBCs pretreated with either PBS, Gln (Q), or QCG were labeled with DCFDA, placed in a glass bottom petri dish per sample condition, and allowed to settle for 45 seconds before given a bolus (red arrow, A) of PBS (gray line, B-D) or 50 μM H2O2 (red line, B-D). Stationary cells (total 8 cells per condition) were quantified (A) and imaged (B-D) per condition at each 15 second time point for 5 minutes. Images were taken with a Leica Thunder at 63× oil immersion (A-D). Representative images of DCFDA labeled RBCs stressed with 50 μM H2O2 (left) or PfCM (right) (E). Images taken with Echo Revolve fluorescent microscope at 100× oil immersion (E).

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