Figure 6.
QCG supplementation rapidly protects RBCs from intracellular ROS formation during oxidative stress. RBCs pretreated with either PBS, Gln (Q), or QCG were labeled with DCFDA, placed in a glass bottom petri dish per sample condition, and allowed to settle for 45 seconds before given a bolus (red arrow, A) of PBS (gray line, B-D) or 50 μM H2O2 (red line, B-D). Stationary cells (total 8 cells per condition) were quantified (A) and imaged (B-D) per condition at each 15 second time point for 5 minutes. Images were taken with a Leica Thunder at 63× oil immersion (A-D). Representative images of DCFDA labeled RBCs stressed with 50 μM H2O2 (left) or PfCM (right) (E). Images taken with Echo Revolve fluorescent microscope at 100× oil immersion (E).