Microfluidic assay determines ex vivo thrombus and NETs formation under arterial flow in vwf^-/- and Adamts13^-/- mice. (A-B) The final mean surface coverage of platelets and leukocytes on the collagen surface and the mean rate of thrombus formation over time, respectively, following perfusion of whole blood prestimulated with Stx-2 under flow from vwf^-/- (top) and WT (bottom) mice, as indicated. (C-D) The final surface coverage of platelets and leukocytes and the rate of thrombus formation over time, respectively, following perfusion of whole blood prestimulated with Stx-2 from WT (top) and Adamts13^-/- (bottom) mice, as indicated. (E-F) Representative confocal microscopic images demonstrate the finally adhered platelets (purple), neutrophils (green), and extracellular DNA strings (elongated green) following perfusion of whole blood samples from vwf^-/- vs WT, and WT vs Adamts13^-/- mice, respectively. (Ei-Fi) are enlarged (5×) areas from the bottom panels of E and F, respectively. Here, a13^-/- is Adamts13^-/-; vwf^-/- is vwf null; n, number of mice; N, neutrophil; PLT, platelets. (G) Quantitation of the number of DNA strings under fluorescent microscope in the blood samples from vwf^-/-, WT, and Adamts13^-/- mice. The data shown in G are individual values (dots), median (bar), and interquartile range (IQR). Kruskal-Wallis test determined the statistical significance among the 3 groups. ∗ indicates P < .05.