Figure 4.
PB responses after experimental vaccination. (A) Representative FACS analysis of gated CD19+ B cells for CD27 and CD38 on days 0 and 7 after vaccination. CD27high CD38high PBs are gated and the percentages of all CD19+ B cells are shown. (B) Summary of PB expansion in all patients. Dose levels of individual patients are depicted in different colors; ∗P < .05. (C) CDR3 clonotype sharing between PBs after experimental vaccination and memory cells from donor–derived B-cell products (left column) and unrelated memory preparations (right column). The color code corresponds to the PB samples. The CDR3 sequences are normalized to 10 million reads per sample. A mean of 1.6 × 105 CDR3 reads was obtained for sorted memory cells (range, 1.1 × 104 to 3.8 × 105), and a mean of 6.8 × 105 CDR3 reads was obtained from sorted PBs from 4 patients after experimental vaccination (range, 3.5 × 105 to 9.7 × 105).

PB responses after experimental vaccination. (A) Representative FACS analysis of gated CD19+ B cells for CD27 and CD38 on days 0 and 7 after vaccination. CD27high CD38high PBs are gated and the percentages of all CD19+ B cells are shown. (B) Summary of PB expansion in all patients. Dose levels of individual patients are depicted in different colors; ∗P < .05. (C) CDR3 clonotype sharing between PBs after experimental vaccination and memory cells from donor–derived B-cell products (left column) and unrelated memory preparations (right column). The color code corresponds to the PB samples. The CDR3 sequences are normalized to 10 million reads per sample. A mean of 1.6 × 105 CDR3 reads was obtained for sorted memory cells (range, 1.1 × 104 to 3.8 × 105), and a mean of 6.8 × 105 CDR3 reads was obtained from sorted PBs from 4 patients after experimental vaccination (range, 3.5 × 105 to 9.7 × 105).

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