Alteration in morphology and reduced growth capacity of MGUS, SMM and MM MSC. (A) Bar charts of CFU-F were normalized to 1 × 107 plated BM-MNC and representative micrograph of the phenotype of MGUS-, SMM- and MM-derived MSC with scale bars indicating 100 μm. (B) Bar charts of the number of senescent cells in native MGUS-, SMM- and MM-derived MSC in passage 3 cells after the β-galactosidase staining and representative micrographs to visualize senescent cells in blue (scale bars indicating 100 μm). For all experiments results are expressed as mean ± SEM. Asterisks indicate P-values ∗P < .05, ∗∗P < .01, ∗∗∗P < .001. (C) Box plot of the TPM (Transcripts per Kilobase Million) values for CDKN2A (p16) from our RNA sequencing data of healthy (n = 5), MGUS (n = 4), SMM (n = 4) and MM (n = 5) MSC. Significances were included from the DESeq2 results of CDKN2A of MGUS, SMM and MM MSC contrasted to healthy MSC. Asterisks indicate P-values ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001.
