RUNX1^R320∗ differential binding is most enriched at enhancer regions. (A-B) Annotation of RUNX1 and RUNX1^R320∗ binding sites using the ChIPSeeker annotation of the hg38 genome for all peaks. Wild-type peaks = 40,679; RUNX1^R320∗ peaks = 38,233. (C) Differential binding volcano plot between RUNX1 wild-type and RUNX1^R320∗ ChIP-seq data sets, significantly upregulated binding shown in (red) and downregulated binding (blue) compared with R320^∗/WT. (D) Analysis of gene expression in RUNX1^R320∗ cells relative to RUNX1 WT for genes with RUNX1 promoter binding. (E) Enrichment of RUNX1^R320∗ peaks genome-wide using ENCODE K562 annotation data across up, nc (no change), and downregulated binding relative to RUNX1 WT. H3K27ac, H3K4me1, H3K4me3, H3K27me3, and H3K9me3 were used to annotate the enhancers, promoters, transcribed regions, repressed regions, and heterochromatin, respectively. (F) RUNX1 motif presence across enhancers and promoters with up- or downregulated binding of RUNX1^R320∗ relative to RUNX1.