Studies performed to dissect the role of NF-ĸB in MPN pathogenesis. Affects of loss of MyD88 and RelA were investigated in the context of Jak2^V617F knockin and MPL^W515L transduction transplantation mouse models. Disruption of miR-146a was investigated in primary patient samples and patient-derived xenografts. The role of IRAK4 in MPN was investigated with primary patient samples, patient-derived xenografts, and mass cytometry. BM, bone marrow; TLR, Toll-like receptor.