![Myd88 ablation alleviates MPN disease burden. (A) Schematic of TLR-mediated NF-κB signaling cascade. (B) Schematic of the Myd88−/−MPLW515L mouse model. (C) WBC counts of MPL (n = 8) and MPL-Myd88−/− (n = 8) mice. Statistics were assessed by 2-way ANOVA. Light gray regions indicate the normal reference range. (D) Monocyte, BM CD45.2 myeloid cells, hematocrit, and platelet counts at end point (MPL [n = 8], MPL-Myd88−/− [n = 7]). Statistics were assessed by 2-tailed Student t test. (E) Normalized spleen weight at end point. Statistics were assessed by 2-tailed Student t test. (F) Percentage of HSCs of BM cells. (G) Distribution of hematopoietic progenitor populations at end point. Statistics were assessed by 2-tailed Student t test. (H) Kaplan-Meier survival curve. Statistics were assessed by log-rank test. (I) Histology of the spleen and BM at end point. Scale bar = 75 μm. Histology of the spleen and BM at end point. Scale bar = 75 μm. BM fibrosis was scored from 3 mice in each group.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/143/23/10.1182_blood.2023022804/2/blood_bld-2023-022804-gr3.jpeg?Expires=1753454277&Signature=lBOJ~j8n79qQPpo~Jv8q6dYVTULC6liaFSzjDIQldFQ3BqjFnmj8M1FTd2In50WCksilAIZhXMn8B8MfG7TtY8mUtQFJP-mVhgup2woGfIrmQz8Brd-WYgHJZRmFbL6pXzZLIHZE~pFV6~fpYX4okLVTw00UqCXICej8Gfy7OsDetbFTTBtF4Sv96~mxA2yMW0CcWty9n-wzDZBLUjwT~d-ysK7HdNPQ964t1lDu1eFwHV-bONGvScKLrAbewO~WHPHEYRiE69CIKnPu-M4pIA4d~GdjaNXVHEO~uFlTQwyI3K1-VwSkJbFaaI7rKAzTyoCBmNiyVyFCUaXnIztu6w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Myd88 ablation alleviates MPN disease burden. (A) Schematic of TLR-mediated NF-κB signaling cascade. (B) Schematic of the Myd88−/−MPLW515L mouse model. (C) WBC counts of MPL (n = 8) and MPL-Myd88−/− (n = 8) mice. Statistics were assessed by 2-way ANOVA. Light gray regions indicate the normal reference range. (D) Monocyte, BM CD45.2 myeloid cells, hematocrit, and platelet counts at end point (MPL [n = 8], MPL-Myd88−/− [n = 7]). Statistics were assessed by 2-tailed Student t test. (E) Normalized spleen weight at end point. Statistics were assessed by 2-tailed Student t test. (F) Percentage of HSCs of BM cells. (G) Distribution of hematopoietic progenitor populations at end point. Statistics were assessed by 2-tailed Student t test. (H) Kaplan-Meier survival curve. Statistics were assessed by log-rank test. (I) Histology of the spleen and BM at end point. Scale bar = 75 μm. Histology of the spleen and BM at end point. Scale bar = 75 μm. BM fibrosis was scored from 3 mice in each group.
