Figure 2.
Effect of ECP therapy on proliferation and long-term cytotoxicity of CAR-T cells in the D model (stimulation by Raji cells). (A) Representative histogram of proliferation of CD4+ and CD8+CAR-T cells with or without ECP therapy after 3 day stimulation by irradiated tumor cells. (B) Statistical analysis of proliferation of CD4+ and CD8+CAR-T cells with or without ECP therapy. (C) The influence of ECP on the expansion of CAR-T cells in a long-term culture assay. The dark curves display the dynamic of tumor cells and the orange and green curves indicate the dynamic of CAR-T cells. The purple arrow represents CAR-T cells and PBMCs in the coculture killing assay, whereas the gray arrow represents tumor cells, which were added once, initially. In addition, the blue arrows represent the quantification of cells in the coculture system every 3 days. (D) The expansion peak of CD3+CAR-T cells (left panel) and the residual tumor cells on the last day of coculture (right panel) in the long-term culture assay. (E) The effect of ECP on the serial killing capacity of CAR-T cells in a challenging assay. The dark curves display the dynamic of tumor cells and the orange and green curves indicate the dynamic of CAR-T cells. The purple arrow represents CAR-T cells and PBMCs in the coculture killing assay, whereas the gray arrow represents tumor cells, which were added in each round. In addition, the blue arrows represent the quantification of cells in the coculture system in every round. (F) The expansion peak of CD3+CAR-T cells (left panel) and the residual tumor cells on the last day of coculture (right panel) in the challenging assay. (G) The effect of ECP therapy on costimulatory marker CD40L expression with or without ECP treatment after 24 hour stimulation with CD19+Raji cells: the frequency of CD40L+CD3+CAR-T cells (left panel) and the density of CD40L expression on CD3+CAR-T cells (right panel). (H) The effect of ECP on expression of the exhaustion marker TIM3 in CD4+ and CD8+CAR-T cells. Samples of 4 individual donors were tested. A 2-tailed t test was used for statistical analysis. ∗P < .05; ∗∗P < .01.

Effect of ECP therapy on proliferation and long-term cytotoxicity of CAR-T cells in the D model (stimulation by Raji cells). (A) Representative histogram of proliferation of CD4+ and CD8+CAR-T cells with or without ECP therapy after 3 day stimulation by irradiated tumor cells. (B) Statistical analysis of proliferation of CD4+ and CD8+CAR-T cells with or without ECP therapy. (C) The influence of ECP on the expansion of CAR-T cells in a long-term culture assay. The dark curves display the dynamic of tumor cells and the orange and green curves indicate the dynamic of CAR-T cells. The purple arrow represents CAR-T cells and PBMCs in the coculture killing assay, whereas the gray arrow represents tumor cells, which were added once, initially. In addition, the blue arrows represent the quantification of cells in the coculture system every 3 days. (D) The expansion peak of CD3+CAR-T cells (left panel) and the residual tumor cells on the last day of coculture (right panel) in the long-term culture assay. (E) The effect of ECP on the serial killing capacity of CAR-T cells in a challenging assay. The dark curves display the dynamic of tumor cells and the orange and green curves indicate the dynamic of CAR-T cells. The purple arrow represents CAR-T cells and PBMCs in the coculture killing assay, whereas the gray arrow represents tumor cells, which were added in each round. In addition, the blue arrows represent the quantification of cells in the coculture system in every round. (F) The expansion peak of CD3+CAR-T cells (left panel) and the residual tumor cells on the last day of coculture (right panel) in the challenging assay. (G) The effect of ECP therapy on costimulatory marker CD40L expression with or without ECP treatment after 24 hour stimulation with CD19+Raji cells: the frequency of CD40L+CD3+CAR-T cells (left panel) and the density of CD40L expression on CD3+CAR-T cells (right panel). (H) The effect of ECP on expression of the exhaustion marker TIM3 in CD4+ and CD8+CAR-T cells. Samples of 4 individual donors were tested. A 2-tailed t test was used for statistical analysis. ∗P < .05; ∗∗P < .01.

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