Alignment of epitope motifs on the linear sequence of FVIII. The plot shows the alignment of 18 epitope motifs on the linear sequence of FVIII. The x-axis represents the linear sequence of FVIII, from position 20 to 2351. The y-axis shows a count for each position of the FVIII sequence. The count for each position was defined as the weighted sum of each cluster whose epitope motif was mapped to that position, using the peptide count of each cluster as weights. For example, if 2 clusters with a peptide count of 20 and 10, respectively, were mapped to a given position, then the total score for that position would be cluster 1 ∗ 20 + cluster 2 ∗ 10 = 30. The number of epitope motifs mapped to each domain, as a proportion of all 18 aligned epitope motifs, is shown at the bottom of the figure.