PD-1⁺ CD8⁺ CAR⁺ T-cell clusters are increased in the CR cohort. (A) Clustered heat map showing key marker expressions on differentially abundant CAR⁺ CD8⁺ clusters. Clusters 8 and 9: PD-1⁺ TCF1⁺. Clusters 7 and 13: PD-1⁺ TCF1^low TOX⁻. Clusters 11 and 12: PD-1⁺ TIM3⁺ T-bet⁺ GZMB⁺. Clusters 2, 3, and 4: PD-1⁻ T-bet⁺ GZMB⁺. Color scale was determined by median normalization of each individual marker with blue representing low expression, white representing median expression, and red representing high expression. (B) Box plots of clusters in CR vs PD cohorts. Cluster abundance was reported as a percentage of CAR⁺ CD8⁺ T cells. (C) UMAP of CAR⁺CD8⁺ T cells in CR vs PD cohorts, colored by cluster. (D) Expression plots of phenotypical and functional markers present on CAR⁺ CD8⁺ T cells. Expression of markers on individual cells were overlaid onto the UMAP space in CR (top) vs PD (bottom) cohorts. Box plots show quartiles with bands at the median; whiskers indicate 1.5 interquartile range; all observations overlaid as dots. P values are from linear regression analysis; ∗P < .05, ∗∗P < .01; ∗∗∗P < .001.