BBB leakage is exacerbated in Jak2^V617F mice after CVST. (A) After CVST, staining for IgG (pink); blood vessel (GLUT-1, green); red blood cells (RBCs, white), and cell nuclei (4′,6-diamidino-2-phenylindole [DAPI], cyan) in brain sections from Jak2^WT and Jak2^V617F mice showed IgG extravasation in hemorrhagic and nonhemorrhagic areas. Scale bars, 25 μm. (B) Bar graph indicated quantification of IgG content (mean ± SD) in whole brain from Jak2^WT and Jak2^V617F mice in sham and CVST conditions at day 1, n = 5 to 6 per group. Data were analyzed using Kruskal-Wallis test followed by post hoc Dunn test; ∗P < .05.