Brain neutrophil infiltration is increased in Jak2^V617F mice after CVST. (A) Representative images of coronal brain sections taken through the area of damage from Jak2^WT and Jak2^V617F mice after CVST at day 1. Hematoxylin and eosin (H&E) staining showed the presence of hemorrhagic (H) lesions and nonhemorrhagic cortical parenchyma (no H) in dotted rectangles. Scale bar, 500 μm. (B) Corresponding brain images of staining for MPO (red); cell nuclei (DAPI, cyan) and red blood cells (RBCs, pink) in “no H” (upper panel) and “H” area (lower panel) from Jak2^WT and Jak2^V617F mice after CVST at day 11. Scale bars, 50 μm. (C) Bar graph indicated number of neutrophils within “no H” cortical parenchyma in sham and CVST mice (Jak2^WT and Jak2^V617F), n = 10 per group. Bar graphs indicated quantification of PF4 (D) and MPO (E) contents in whole brain from Jak2^WT and Jak2^V617F mice at day 1 in sham-operated and CVST mice, n = 5 to 6 per group. All results are expressed as mean ± SD. Data were analyzed by Kruskal-Wallis test followed by post hoc Dunn test; ∗P < .05 and ∗∗∗P < .01.