Increased formation of PNAs in Jak2^V617F mice. (A) Circulating PNAs and (B) neutrophil CD11b expression are measured in Jak2^WT and Jak2^V617F mice at baseline, and at day 1 in sham-operated and CVST mice. (C) MPO and (D) H3Cit-DNA plasma levels are measured in the same conditions. PNAs were calculated from Ly6G-positive events as the percentage of neutrophils. Results are expressed as mean ± SD, n = 5 to 6 per group. Data were analyzed by Kruskal-Wallis test followed by post hoc Dunn test. ∗P < .05; ∗∗P < .01. D1, day 1.