Figure 4.
YY1 inhibits SMC3 expression directly. (A) ChIP-seq binding profile for YY1 at the Smc3 locus in mESCs and YY1 ChIP-qPCR primer design strategy at the Smc3 promoter (supplemental Figure 10). (B) Yy1 and Smc3 transcript levels in HPC7 and total BMCs with and without ectopic YY1 expression. (C) ChIP-qPCR analysis of YY1 bindings at the Smc3 promoter in HPC7 cells infected with MigR1-YY1 or MigR1 vector only. (D) quantitative reverse transcription PCR to detect transcript levels in Lin− BMCs of Yy1−/−and Yy1+/+ mice. Primers are listed in supplemental Figure 10. (E) Western blot and quantification to detect the YY1, SMC3, and RAD21 protein expressions in total BMCs of Yy1−/−and Yy1+/+ mice. (F) ChIP-qPCR analysis of the binding of YY1 at the Smc3 promoter region in Yy1+/+ and Yy1−/−total BMCs. Data are presented as means ± standard deviation (SD); ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.

YY1 inhibits SMC3 expression directly. (A) ChIP-seq binding profile for YY1 at the Smc3 locus in mESCs and YY1 ChIP-qPCR primer design strategy at the Smc3 promoter (supplemental Figure 10). (B) Yy1 and Smc3 transcript levels in HPC7 and total BMCs with and without ectopic YY1 expression. (C) ChIP-qPCR analysis of YY1 bindings at the Smc3 promoter in HPC7 cells infected with MigR1-YY1 or MigR1 vector only. (D) quantitative reverse transcription PCR to detect transcript levels in Lin− BMCs of Yy1−/−and Yy1+/+ mice. Primers are listed in supplemental Figure 10. (E) Western blot and quantification to detect the YY1, SMC3, and RAD21 protein expressions in total BMCs of Yy1−/−and Yy1+/+ mice. (F) ChIP-qPCR analysis of the binding of YY1 at the Smc3 promoter region in Yy1+/+ and Yy1−/−total BMCs. Data are presented as means ± standard deviation (SD); ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.

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