SOX11–associated BL signature found in transduced cell lines is also detected in BL primary cases. (A) Western blot experiment showing the SOX11 protein levels in Ramos SOX11⁺ (SOX11 is FLAG-tagged) cell line, Ramos CT, BL2 CT, and BL2-SOX11KO BL cell lines. Tubulin protein was used as loading control. (B) Overlap between DEGs (adjusted P value <.1 and absolute log₂-transformed fold change of >0.65) in DG75 ER-SOX11 vs DG75 ER (in purple, 1694 genes; supplemental Table 6), BL2 CT vs BL2-SOX11KO (in red, 107 genes; supplemental Table 8), and Ramos SOX11⁺ vs Ramos CT (in green, 151 genes; supplemental Table 7). (C) Row scaled expression (Z score) of genes from the SOX11–associated BL signature (79 DEGs overlapping between at least 2 comparisons in Figure 5B) in RNA-seq data of 117 pediatric eBLs and sBLs. K-means clustering was performed to separate samples in k = 3 groups. SOX11 mRNa expression, EBV status, and BL epidemiological variant are shown at the top panel. (D) Correlation plot between genes from the SOX11–associated BL signature in RNA-seq data of 117 pediatric eBLs and sBLs. Blue and red showed positive and negative Pearson correlation coefficients, respectively. P values from Pearson correlation are shown: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001.