Figure 1.
NSD2 overexpression drives metabolic dependencies in MM. (A) Immunoblot analysis confirming the decrease in NSD2 levels and H3K36me2 levels in TKO compared to NTKO isogenic MM cells derived from KMS11. (B) Workflow of genome wide CRISPR screening in NTKO and TKO MM cells. (C-D) MAGeCK-generated gene essentiality (Beta) scores plotted vs gene ranks. Essential genes for NTKO and TKO cells (MAGeCK FDR < 0.1) are indicated in red and blue, respectively. (E) Venn diagram showing common and differentially essential genes between NTKO and TKO cells. (F) Scatter plot of NTKO and TKO Beta scores. Genes that are more essential for NTKO cells (difference in Beta scores > 0.5; FDR < 0.1) are shown in red. (G-I) ShinyGO gene ontology (GO) analyses of the 282 genes defined to be more essential in NTKO than TKO cells. FDRs of the significant GO terms are plotted against the corresponding number of gene hits in each term.

NSD2 overexpression drives metabolic dependencies in MM. (A) Immunoblot analysis confirming the decrease in NSD2 levels and H3K36me2 levels in TKO compared to NTKO isogenic MM cells derived from KMS11. (B) Workflow of genome wide CRISPR screening in NTKO and TKO MM cells. (C-D) MAGeCK-generated gene essentiality (Beta) scores plotted vs gene ranks. Essential genes for NTKO and TKO cells (MAGeCK FDR < 0.1) are indicated in red and blue, respectively. (E) Venn diagram showing common and differentially essential genes between NTKO and TKO cells. (F) Scatter plot of NTKO and TKO Beta scores. Genes that are more essential for NTKO cells (difference in Beta scores > 0.5; FDR < 0.1) are shown in red. (G-I) ShinyGO gene ontology (GO) analyses of the 282 genes defined to be more essential in NTKO than TKO cells. FDRs of the significant GO terms are plotted against the corresponding number of gene hits in each term.

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