Neonatal nNIF inhibits MET formation in J774 cells and human MDMs. J774 cells and MDMs were preincubated with medium alone, nNIF (1 nM), or nNIF-scramble (1 nM) for 1 hour. J774 cells were then stimulated with LPS (100 ng/mL, 4 hours) and MDMs stimulated with E coli (MOI 5, 4 hours). (A-B) Representative live cell images obtained at 60× original magnification with confocal microscopy of MET formation (METs, magenta; nuclear DNA, green) in J774 cells (A) and MDMs (B). (C-D) Fluorometric quantification of MET-associated extracellular DNA in J774 cells after LPS (C) and E coli stimulus (D). (E) Fluorometric quantification of MET-associated extracellular DNA in MDMs after E coli stimulus. Fluorescence intensity shown on the y-axis (mean ± standard error of the mean [SEM]), with each treatment group shown on the x-axis. (F-G) Supernatant CitH3 concentration in J774 cells (F) and MDMs (G) after E coli stimulus. CitH3 concentration shown on the y-axis (mean ± SEM), with each treatment group shown on the x-axis. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. RFU, relaive fluorescence units.