T-bet is required for interferon signaling in CLL cells. (A) GSEA of RNA-seq of Tbx21–/– (n = 6) vs Tbx21+/+ (n = 6) TCL1 cells and TBX21low vs TBX21high CLL cells was performed, and commonly regulated gene sets are depicted. (B) KEGG pathway analysis of RNA-seq and MS data of T-betlow vs T-bethigh CLL cells. (C) Basal expression of ISGs in Tbx21–/– compared with Tbx21+/+ TCL1 cells, as analyzed by RNA-seq. (D) Purified Tbx21–/– and Tbx21+/+ TCL1 cells were stimulated in vitro with IFNβ. Log2FC of ISG expression in comparison with the medium control, as analyzed by quantitative reverse transcription polymerase chain reaction. P values were obtained by multiple t tests and controlling the FDR using the BH method. NES, normalized enrichment score. ∗P ≤ .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.
