Pseudotemporal analysis of prePB and PB subpopulations. (A) UMAP representation of proliferative prePB and PB. (B) UMAP projection colored by normalized pseudotime analysis. (C) Clusters identified using the Monocle 3 package and used to define trajectories. (D) Temporal gene expression patterns from prePB to PB. (E) Proportion of genes coding TFs, epigenetic enzymes (EEs) and proteins involved in ligand/receptor interactions deregulated along the trajectory according to the expression patterns defined in panel D. (F) Plots of the expression of top differentially expressed genes coding for TFs, EEs, ligands and receptors in function of pseudotime.