Combined deletion of PDK2/4 results in reduced neutrophil-mediated platelet aggregation. The extent of (A) cathepsin G and (B) elastase release was measured using enzyme-linked immunosorbent assay in WT or PDK2/4^–/– neutrophils that were stimulated with PMA (50 nM) for 30 minutes. Values are mean ± SEM, n = 5 mice per group. Statistical analysis was performed using 2-way ANOVA followed by Tukey multiple comparisons test. (C) WT platelets were coincubated and stirred with WT or PDK2/4^–/– neutrophils in an aggregometer before the addition of PMA (10 nM). Results are expressed as the percent change in light transmission with respect to the blank (buffer without platelets and neutrophils) set at 100%. Left: the representative aggregation curve is shown. Right: the line graph shows the quantified data for platelet aggregation. Values are mean ± SEM, n = 5 mice per group. Statistical analysis was performed using 2-way ANOVA followed by Šidák multiple comparisons test. Neu, neutrophils; Plt, platelets.