Deletion of PDK2/4 inhibits aerobic glycolysis in PMA-stimulated neutrophils. The proton efflux rate (PER) and glycoPER were measured in PMA (100 nM)–stimulated WT or PDK2/4^−/− neutrophils using glycolytic rate assay. The line graph represents the PER, and compensatory glycoPER (after the sequential addition of mitochondrial inhibitors, rotenone and antimycin A [AA]). The middle and right panels show the quantification of PER and glycoPER. The bar graph shows the quantified data. Values are mean ± SEM, with n = 4 to 5 mice per group. Statistical analysis was performed using 2-way ANOVA followed by Tukey multiple comparisons test.