Competition of Affimers with Nb2 for GPVI dimer binding. (A) Displacement of Affimers by Nb2-GPVI dimer binding in competition ELISA. Affimers M17, D22, and D18 (2 μM) were incubated with 100 nM Nb2 before the addition to immobilized GPVI dimer. (B) Effect of Affimers at multiple concentrations on Nb2-GPVI dimer binding. Affimer scaffold was used as control. Data were presented as mean ± SD; n = 3.