Figure 5.
![Thromboelastograms obtained by ROTEM in HA mice infused with D519V/E665V/K1813A and D519V/E665V FVIII mutants. WT-FVIII (2 μg/kg), mutant; D519V/E665V/K1813A (0.25, 0.5, 1 and 2 μg/kg), or D519V/E665V (1 μg/kg) was infused to the HA mice. Citrated whole-blood samples taken from these mice were assessed by a ROTEM triggered by CaCl2, as described in “Methods.” A representative thromboelastogram is shown. The average parameters (CT, clot formation time [CFT], CT + CFT, α angle, and maximum clot firmness [MCF]) in WT mice and HA mice infused with each FVIII mutant are shown below each figure.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/8/15/10.1182_bloodadvances.2023012391/2/blooda_adv-2023-012391-gr5.jpeg?Expires=1735548919&Signature=TLfWO2fmBYZKhIQO3dOBaQ3Jo1s-1U~KcU~G8~Z5-wR6h4o29YHYe0tiJgQWffZO~Q7E6T0xK8j7M1wG5Gr-4y92mNHDJYb-zXPTxE9Hmv6cTht9bl5~R0XTunJ79fGmfwrd3uXha7f7dqXlAGxaFgfUuDHESRz6z8T12fzA-GRxS6WMYtECNw-H-21CjTYPT-qEHRfYd12CrSbz6e~ORJezGWlWSk3jM-5gC9vceNsjMUsPtDP50yCzcnzF1m2ocMTWYMM2BeTazquu-x8gG6GXimOV4u0Hz~m~exh01gt4ZAge7w2E4zsIzV232-3C3ak0LHXysJOspEQJKwmLEg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Thromboelastograms obtained by ROTEM in HA mice infused with D519V/E665V/K1813A and D519V/E665V FVIII mutants. WT-FVIII (2 μg/kg), mutant; D519V/E665V/K1813A (0.25, 0.5, 1 and 2 μg/kg), or D519V/E665V (1 μg/kg) was infused to the HA mice. Citrated whole-blood samples taken from these mice were assessed by a ROTEM triggered by CaCl2, as described in “Methods.” A representative thromboelastogram is shown. The average parameters (CT, clot formation time [CFT], CT + CFT, α angle, and maximum clot firmness [MCF]) in WT mice and HA mice infused with each FVIII mutant are shown below each figure.
