Mitoxantrone relieved anemia in β-thalassemic mice. (A) Representative pictures (scale bar, 20 μm) of circulating erythrocytes, stained with brilliant cresyl blue. (B) Statistics of reticulocyte identified by brilliant cresyl blue staining. Standard hematological tests of RBC counts (C), hemoglobin concentration (HGB; D), hematocrit (HCT; E), and reticulocyte ratio (F). (G) Plasma EPO concentration evaluated by enzyme-linked immunosorbent assay. The groups mean WT and Hbb^Th3/+ (Th3/+) mice treated with either mitoxantrone (Mito) or PBS. The bar plot represents the mean ± standard deviation (SD) of samples (n = 6-8 mice per group). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.