Mitoxantrone alleviated expanded erythropoiesis in β-thalassemic mice. (A-B) Representative images (A) and weight statistics of spleens (B). Percentages of Ter119⁺ cells assayed by flow cytometry in bone marrow (C) and spleens (D). Erfe mRNA levels detected by qRT-PCR in bone marrow (E) and spleens (F). The groups mean WT and Hbb^Th3/+ (Th3/+) mice treated with either Mito or PBS. The bar plot represents the mean ± SD of samples (n = 6-8 mice per group). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.