Mitoxantrone improved ineffective erythropoiesis in β-thalassemic mice. Representative density plots (A) of flow cytometry analysis with CD44 (y-axis)/Ter119/FSC (x-axis) as markers of nucleated (red) and enucleated (black) erythroid cells and statistics (B) in bone marrow Ter119⁺ cells. Representative density plots (C) of flow cytometry analysis with CD44 (y-axis)/Ter119/FSC (x-axis) as markers of nucleated (red) and enucleated (black) erythroid cells and statistics (D) in spleen Ter119⁺ cells. Representative image (E) and densitometry quantification (F) of α-globin precipitation on circulation erythrocyte membrane via triton-acetic acid-urea gel analysis. The groups mean WT and Hbb^Th3/+ (Th3/+) mice treated with either Mito or PBS. The bar plot represents the mean ± SD of samples (n = 6-8 mice per group). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. FSC, forward scatter.