Clinical demographics of patients with MPN and healthy controls. (A) Similarity in distribution of MPN subtypes and controls, with slightly higher proportion ET (PV n = 41, ET n = 59, control n = 40). (B) Comparable and balanced distribution of sex across MPN subtypes and controls. Larger percentage of female healthy controls. (C) All patients with PV harbored the JAK2 V617F mutation, and in keeping with the general ET population JAK2 V617F was the most common driver mutation followed by CALR and MPL, with 12 patients with triple-negative ET included in this study. (D) MPN patient therapies reflecting current clinical practice. Most patients with PV and ET were prescribed aspirin (ASA), with hydroxyurea (HU) as a commonly used cytoreductive therapy. To control for any interpatient variability, all treatment, in addition to patient, sex and experimental batch are adjusted as confounding factors in downstream differential expression analyses. (E) Comparable distribution of age across MPN subtypes and controls. Violin plots of patient age from each MPN subtype reflect clinical expectation, with slightly higher median age noted for patients with ET and PV than that for controls. (F) Platelet counts, as box plots, measured at the same date and time as experimental platelet sampling. As expected, Mann-Whitney U tests marked by asterisks indicate a statistically significant difference between control and MPN groups (∗∗∗∗P ≤ 0.0001; ns, not significant).