Figure 3.
Effect of EDTA treatment on R21D10 binding to αIIbβ3. Washed platelets were treated with 10 mM EDTA for 30 minutes at pH 7.4 and room temperature (A-B), at pH 8.0 and 37°C (C-D), and then the binding of Alexa Fluor 488–labeled R21D10 or 10E5 was detected by flow cytometry.

Effect of EDTA treatment on R21D10 binding to αIIbβ3. Washed platelets were treated with 10 mM EDTA for 30 minutes at pH 7.4 and room temperature (A-B), at pH 8.0 and 37°C (C-D), and then the binding of Alexa Fluor 488–labeled R21D10 or 10E5 was detected by flow cytometry.

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