Increased fibrin accumulation at sites of hemostatic plug formation in plasminogen-deficient mice. (A) Representative images for fibrin (blue) and platelet (gray) accumulation at the indicated time points after laser injury in WT (left panel) and Plg^−/−mice (right panel). (B) SFI ± SEM for fibrin signal. (Plg^−/−, n = 15 injuries in 4 mice; and WT, n = 19 injuries in 3 mice). Same controls as in Figure 2F. (C) Fibrin area coverage (μm²). (D) Fibrin ring thickness (μm) measured at t = 13 minutes after laser injury. (E) Overlay of fluorescence intensity histograms for platelets (gray) and fibrin (blue) in plugs from a WT (left panel) or Plg^−/− mouse (right panel), measured along 1 cross-section slice of a Z-max projection. Statistical significance was determined for fluorescence signals of the last 3D stack acquired during the observation period. ∗∗∗P < .001. Scale bar indicates 50 μm.