Validation of CML BCR::ABL1 SHERLOCK on dried blood spot samples. (A) Schematic diagram illustrating the sources of dried blood spot samples and approach to testing by isoform-agnostic CML BCR::ABL1 SHERLOCK. Red points indicate sample country of origin, and size of point reflects number of samples from country. (B) Background-subtracted fluorescence from isoform-agnostic CML BCR::ABL1 SHERLOCK performed on diagnostic BCR::ABL1 RT-PCR–positive CML dried blood spots samples (n = 44) and BCR::ABL1 RT-PCR–negative controls (n = 15). (C) Summary concordance of isoform-agnostic CML BCR::ABL1 SHERLOCK results and BCR::ABL1 RT-PCR in field-based dried blood spot CML and control samples.