BCR::ABL1 p190 SHERLOCK assay for Ph⁺ ALL. (A) Prevalence of BCR::ABL1 p210 and p190 transcripts in Ph⁺ ALL. (B) Illustration of BCR::ABL1 p190 e1a2 assay design strategy with fusion-specific RT-RPA primers and junction-specific crRNA guides. (C) LOD for BCR::ABL1 p190 synthetic target. (D) Background-subtracted mean fluorescence intensity for serial RNA samples from patients with Ph⁺ ALL and controls. Corresponding RT-qPCR transcript %IS noted for each SHERLOCK measurement.