Accumulation of late-stage inflamed NK cells in MM. (A) Trajectory analysis results overlaid on UMAP embeddings identified 3 lineages seeding from CD56^bright NK cluster 5. (B) Branching of the minimum spanning tree built at the cluster level differentiates BM-resident NKs, terminal NKs, and inflammatory MM-enriched NK cells. (C) Cell density distributions along trajectory pseudotime describes NK cells differentiation. (D) Pseudotime distributions of PB and BM NK cells from patients with MM and HDs. (E) Trajectory pseudotime correlation with MELD MM vs HD likelihood. (F) Gene expression along pseudotime trajectory. (G) Comparison of gene expression programs between cluster 3 and 1 using GSEA analysis of KEGG gene sets. (H) Comparisons between GSEA normalized enrichment scores (NES) of C7 vs C4 and C3 vs C1 NK clusters. (I) An inflamed NK cell signature was generated with genes common to clusters C7 and C3 (see supplemental Table 5). UMAP and graphs showing its relative expression of in PB or BM HD and MM NK cells.