mRNA expression levels of CLU and its isoforms CLU1 and CLU2 in platelets from groups of patients with ITP compared with healthy controls. Total RNA from platelets was isolated and subjected to qRT-PCR with 18S ribosomal RNA as internal control. Relative mRNA levels of each sample normalized to healthy controls are shown as mean ± standard deviation (SD). Statistical analyses were performed using 1-way analysis of variance (ANOVA) followed by multiple comparisons tests to compare the mean ranks between the groups. Means are significantly different with P < .001 (highly significant).