Molecular validation of enhancer-promoter interaction in ChiPSC22^t(7;12) upon differentiation. (A) Scheme of the enhancer deletion experiment performed to validate the interaction between the MNX1 promoter and enhancers distal to the BP. (B) Gene expression in HSPCs derived from ChiPSC22^WT (n = 3), ChiPSC22^t(7;12) (n = 4, from 2 independent cell lines), and ChiPSC22^t(7;12)ΔEn (n = 3, from 2 independent cell lines) measured via qRT-PCR and shown as 2^−ΔCt vs GUSB as endogenous reference. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05. ns, not significant.